| Team |
Country |
Qualifying Method |
| América |
Mexico |
Interliga 2008 winner |
| Arsenal |
Argentina |
5th place in 2006-07 season |
| Atlético Nacional |
Colombia |
Apertura 2007 winner & Finalización 2007 winner |
| Audax Italiano |
Chile |
1st place Clausura league stage 2007 |
| Boca Juniors |
Argentina |
Copa Libertadores 2007 winner |
| Boyacá Chicó |
Colombia |
Second best-place finisher 2007 season |
| Caracas FC |
Venezuela |
Apertura 2006 winner |
| Cerro Porteño |
Paraguay |
Best-place finisher 2007 season |
| CF Atlas |
Mexico |
Interliga 2008 runner-up |
| Cienciano |
Perú |
Best-place finisher 2007 season |
| Colo-Colo |
Chile |
Apertura 2007 winner & Clausura 2007 winner |
| Coronel Bolognesi |
Perú |
Clausura 2007 winner |
| Cruzeiro |
Brazil |
Campeonato Brasileiro Serie A 2007 5th place |
| Cúcuta Deportivo |
Colombia |
Best-place finisher 2007 season |
| Danubio F.C. |
Uruguay |
2006-07 winner |
| Deportivo Cuenca |
Ecuador |
Liguilla Final 2007 runner-up |
| Deportivo Olmedo |
Ecuador |
Liguilla Final 2007 3rd place |
| Estudiantes |
Argentina |
Apertura 2006 winner |
| Flamengo |
Brazil |
Campeonato Brasileiro Serie A 2007 3rd place |
| Fluminense |
Brazil |
Copa do Brasil 2007 winner & Campeonato Brasileiro Seria A 2007 4th place |
| Guadalajara |
Mexico |
Apertura 2006 winner |
| La Paz |
Bolivia |
Apertura and Clausura Runners-up playoff winner |
| Lanús |
Argentina |
6th place in 2006-07 season |
| LDU |
Ecuador |
Liguilla Final 2007 winner |
| Libertad |
Paraguay |
Clausura 2007 winner |
| Mineiros de Guayana |
Venezuela |
Best-place finisher 2007-06 season |
| Montevideo Wanderers |
Uruguay |
Liguilla Pre-Libertadores 2007 runner-up |
| Nacional |
Uruguay |
Liguilla Pre-Libertadores 2007 winner |
| Real Potosí |
Bolivia |
Apertura 2007 winner |
| River Plate |
Argentina |
4th place in 2006-07 season |
| San José |
Bolivia |
Clausura 2007 winner |
| San Lorenzo |
Argentina |
Clausura 2007 winner |
| Santos |
Brazil |
Campeonato Brasileiro Serie A 2007 2nd place |
| Sportivo Luqueño |
Paraguay |
Apertura 2007 winner |
| São Paulo |
Brazil |
Campeonato Brasieiro Serie A 2007 winner |
| UA Maracaibo |
Venezuela |
Clausura 2007 winner |
| Universidad Católica |
Chile |
2nd place Apertura league stage 2007 |
| Universidad San Martín |
Perú |
Apertura 2007 winner |